Professional hunter, Theo Bronkhorst and farm owner, Honest Ndlovu, are charged with poaching offences for not having the required hunting permit.
United States (U.S.) dentist, Walter Palmer, shot the animal with a bow and rifle.
Mr. Palmer, who says he was unaware of the lion’s identity, paid for the hunt and could also face poaching charges.
The two Zimbabwean men, who accompanied him on the hunt, could face up to 15 years in prison if found guilty.
Mr. Palmer says he regrets shooting the well-known animal and believed he was on a legal hunt. He relied on professional guides to find a lion and obtain the necessary permits, he said.
