The male nurse from Sardinia spent a month receiving treatment at the Spallanzani infectious diseases clinic in Rome.
He was the second Italian to contract the disease after a doctor who had also worked for Emergency in Sierra Leone.
The doctor, who left hospital in January, was treated at the same clinic with a combination of experimental drugs and the blood plasma of an Ebola survivor.
The health ministry said the nurse had been treated with drugs sourced overseas but did not reveal further details.
Ebola has killed at least 11,000 people since its latest outbreak in West Africa started in December 2013.
